KMID : 0613820100200050729
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Journal of Life Science 2010 Volume.20 No. 5 p.729 ~ p.735
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A Study on the Gene Expression of Adipogenic Regulators by an Herbal Composition
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Lee Hae-Yyong
Park Suk-Won Oh Dong-Jin Chae Soo-Ahn Lee Jung-Ju Yoon Yoo-Sik Kang Ryun-Hwa Bae Sung-Min Cho Soo-Hyun Shim Yae-Jie
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Abstract
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In our previous study, it was reported that an herbal mixture, SH21B, inhibits fat accumulation and adipogenesis both in vitro and in vivo models of obesity. SH21B is a mixture composed of seven herbs: Scutellaria baicalensis Georgi, Prunus armeniaca Maxim, Ephedra sinica Stapf, Acorus gramineus Soland, Typha orientalis Presl, Polygala tenuifolia Willd, and Nelumbo nucifera Gaertner (Ratio 3:3:3:3:3:2:2). The aim of this study was to investigate the detailed molecular mechanisms of the effects of SH21B on various regulators of the adipogenesis pathway. During the adipogenesis of 3T3-L1 cells, SH21B significantly decreased the expression levels of central transcription factors of adipogenesis, such as peroxisome proliferator-activated receptor (PPAR)¥ã and CCAAT/enhancer binding protein (C/EBP)¥á. To elucidate the detailed molecular mechanism of the anti-adipogenic effects of SH21B, we examined the expression levels of the various pro-adipogenic or anti-adipogenic regulators of adipogenesis upstream of PPAR¥ã and C/EBP¥á. The mRNA levels of Krox20 and Kruppel-like factor (KLF) 15, which are pro-adipogenic regulators, were significantly down-regulated by SH21B treatment, whereas the mRNA levels of C/EBP¥â and KLF5 were not changed. KLF2 and C/EBP homologous protein (CHOP), which are anti-adipogenic regulators, were significantly up-regulated by SH21B treatment. These results suggest that the molecular mechanism of the anti-adipogenic effect of SH21B involves both the down-regulations of pro-adipogenic regulators, such as Krox20 and KLF15, and the up-regulations of anti-adipogenic regulators, such as KLF2 and CHOP, which results in the suppression of central transcription factors of adipogenesis including PPAR¥ã and C/EBP¥á.
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KEYWORD
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Adipogenesis, SH21B, Krox20, KLF15, KLF2, CHOP
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